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Brain Tissue Fixed by Focused Beam Microwave Veloz-Castillo et al. · KAUST × NICO Turin · volume EM
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GLYCOGEN

Glycogen granules

fixation—
model size—
segmented bydeep learning
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Fixation method
Structure
KAUST Visualization Core Lab

Electron Microscopy of Brain Tissue Fixed by Focused Beam Microwave to Preserve Glycogen

María Fernanda Veloz-Castillo, Ronell Sicat, Christel Genoud, Pierre J. Magistretti, Corrado Calì
WhyGlycogen — the brain's main energy reserve — degrades rapidly post-mortem. Conventional chemical fixation fails to preserve its native levels, so this study uses focused beam microwave (FBMW) fixation as a rapid euthanasia + fixation method that better reflects in vivo conditions.
ConditionsPerfused = conventional PFA+GA perfusion (baseline). 4 kW · 600 ms and 5 kW · 600 ms = focused microwave irradiation settings applied at the head.
PipelineVolume EM stacks (Zeiss Volumescope) → manual + deep-learning segmentation (MitoNet, ResNet-Cardona, VGG19, DeepLab) → 3D reconstruction.
PresentedGordon Research Seminar on Volume Electron Microscopy, Barcelona, May 2025.
This demoThe 3D reconstructions from the poster, delivered in the phone browser — no app install. Models compressed from 85 MB to 2.5 MB total for instant loading.